Hypoxia-inducible factor Essay Example | Topics and Well Written Essays - 1250 words

Hypoxia-inducible factor - Essay ExampleHypoxia is a condition where physiologic type O levels fall dismantle than the normal, which can result in stroke, brain injury, spinal cord injury, other neurodegenerative diseases, and cancer. Because of the importance of group O for life, organisms have developed mechanisms to cope and survive low oxygen levels (hypoxia). During hypoxia, cells adapt by altering the expression of many genes those involved in maintaining oxygen homeostasis, coping with reactive oxygen species and other aftermaths of low oxygen stress. Many of these genes are directly regulated by the hypoxia-inducible transcription factor (HIF with common isoforms HIF-1 and HIF-2). When oxygen levels are normal (normoxia), HIF is barely discernible under hypoxia the HIF stringency increases dramatically. The active form of HIF is composed of two sub-units, HIF , and HIF . The latter is constitutively expressed regardless of physiologic oxygen concentration, while HIF conce ntration is very low under normoxic conditions further increases with hypoxia. In normoxia, HIF is hydroxylated by through the action of prolyl hydroxylases domain proteins or PHD. Hydroxylation allows HIF to associate with a protein complex that makes it a target for proteolytic degradation. The proof of the inhibitory effect of PHD on HIF was established when silencing of PHD2 gene increased normoxic HIF levels (Berraet al., 2003). Silencing of other identified PHD, 1 and 3, did not affect normoxic levels of HIF, leading to the conclusion that the PHDs have different roles in vivo (Berra et al., 2003).